Lab-Grown Organoids Could Transform Female Reproductive Medicine
Artificial tissues that mimic the placenta, endometrium, ovary and vagina could point to treatments for common conditions such as preeclampsia and endometriosis
In 2017, Ashley Moffett, a reproductive immunologist, walked to the pharmacy near her laboratory at the University of Cambridge, UK, to buy a pregnancy test. But it wasn’t for Moffett. Her postdoc, Margherita Turco, had created what she thought might be the first cluster of cells capable of mimicking the tissue of the placenta — a placental organoid. But she needed a way to be sure.“We must do a pregnancy test on them,” Moffett said.If Turco was correct, the miniature ball of cells she had created would secrete HCG, the hormone that triggers a positive pregnancy test. “I took the stick, put it in, and it was positive,” says Turco, now a reproductive biologist at the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland. “It was the best celebration.”On supporting science journalismIf you're enjoying this article, consider supporting our award-winning journalism by subscribing. By purchasing a subscription you are helping to ensure the future of impactful stories about the discoveries and ideas shaping our world today.Scientists make organoids such as this by coaxing stem cells to grow in a jelly-like substance and to self-assemble into clumps of tissue. The typically hollow or solid balls of cells don’t look anything like real organs. But they do take on key aspects of the organ that they’re meant to represent — liver, brain, lung or stomach, for instance.The mini-organs have the advantage of being more realistic than a 2D cell culture — the conventional in vitro workhorses — because they behave more like tissue. The cells divide, differentiate, communicate, respond to their environment and, just like in a real organ, die. And, because they contain human cells, they can be more representative than many animal models. “Animals are good models in the generalities, but they start to fall down in the particulars,” says Linda Griffith, a biological engineer at the Massachusetts Institute of Technology in Cambridge.Over the past decade, organoid research has exploded. Researchers have used them to study early brain development, test cancer therapies and much more. And these 3D models stand to become even more crucial as US agencies, including the National Institutes of Health, the Food and Drug Administration and the Environmental Protection Agency, aim to move away from animal testing.Now researchers are using organoids to study female reproduction, an area in which animal models can be especially limited. Lab mice, for example, don’t menstruate. And their placentas don’t develop in the same way as human placentas do. That challenge, along with a historical lack of funding for women’s health research, has left basic questions unanswered.“I really see it as a powerful model to do science,” says Mirjana Kessler, a cell biologist at the Ludwig Maximilian University of Munich in Germany, who has developed an organoid that mimics the fallopian tube and a biobank of ovarian cancer organoids.Organoids of the placenta, endometrium, ovary and vagina could help to reveal how these organs function, and what happens when things go awry.“There’s so much work to do to understand the normal biology,” Turco says.The placenta invadesThe placenta plays a key part in maternal health during pregnancy. Humans aren’t the only species that develops a placenta, but the “human placenta is quite different than most other species, even primates actually, apart from apes”, says Moffett. Mice and humans, for example, both have placentas that invade the uterine lining, but the timing of development and the depth of invasion differ. Exactly what happens during the early days of placental development is still unclear, but problems at this stage can have serious consequences later.One of the placenta’s first jobs is to create a link between the mother and the developing embryo. To do this, the placenta invades the spiral arteries that feed the uterus. The invasive cells open up the arteries, “essentially making a channel so that mom can provide what she needs through her blood supply”, says Victoria Roberts, a developmental biologist at the Oregon National Primate Research Center in Beaverton. (Nature recognizes that transgender men and non-binary people might have female reproductive organs and might become pregnant. ‘Mother’ is used in this article to reflect language used by the field.)The process can be deadly if it goes wrong. If the placenta invades too deeply, a condition called placenta accreta, the expectant mother can lose too much blood during birth. And if the organ doesn’t invade deeply enough, then the fetus might not get enough nutrients to sustain its growth.Organoids made of placental cells can help reveal how the organ invades the uterine lining.Turco lab, Friedrich Miescher Institute for Biomedical ResearchShallow invasion can also impact the mother’s health. When the placenta doesn’t get enough blood, research suggests it can become inflamed and secrete harmful factors into the mother’s blood that trigger pre-eclampsia, a condition characterized by protein build-up in the blood and dangerously high blood pressure. Worldwide, 2–8% of pregnant people develop the condition. “It’s a very serious pregnancy complication that goes silent and undetected until very late into pregnancy,” says Quinton Smith, a chemical engineer at the University of California, Irvine. The only way to cure the condition is to deliver the baby, even if that means a preterm birth.To better understand the condition, Smith, Turco and other researchers are using organoids made of placental cells called trophoblasts to model the molecular processes involved. Turco is focused on the basic biology of how invasion is regulated, a process that seems to be controlled by both the fetus and the mother. “It’s got to be a compromise,” Moffett says. “It’s an absolute dialogue.”That dialogue seems to be happening between the placenta and the uterine lining. As a case in point, when an embryo implants somewhere the lining doesn’t exist — on a scar left by a previous caesarean delivery or in a fallopian tube, for example — “there’s no control of the invasion at all”, Turco says.Research suggests that immune cells called uterine natural killer cells have a key role in this conversation. The cells don’t kill but instead send out chemical signals that help to regulate the invasion of the uterine lining.When Turco, Moffett and their colleagues exposed the mini-placentas to these chemical signals and analysed which genes the cells expressed, they found that many were associated with pre-eclampsia.“I’m sure it’s not the whole story,” Moffett says. “But it does show you how you can use those organoids to ask these fundamental questions about human pregnancy.”Mimicking menstruationTurco’s first attempt to create a mini-placenta in 2016 didn’t go as planned. The placental tissue she was working with contained not only trophoblasts, but also a few rogue maternal cells from the endometrium, the uterine lining that builds up and then sheds each month during menstruation. Those maternal cells “kept on growing and taking over,” she says. “It was a setback at that time.”But now Turco sees it as a wonderful discovery, because she instead grew organoids that represent the endometrium. This, along with another endometrial model published in the same year, really opened the door for everyone else, says Griffith.Griffith has been studying the endometrium for more than a decade. The research is personal. When Griffith hit puberty, she developed a debilitating condition called endometriosis. The disease, which affects about 10% of people with a uterus who are of reproductive age, occurs when endometrium-like tissue grows in places it doesn’t belong.Because this tissue is trapped inside the body, it can’t be shed properly. Instead, it can irritate surrounding healthy tissue, causing inflammation, pain and scar tissue. Although existing therapies address some of the symptoms, they don’t provide a cure.Organoids are typically grown in Matrigel, a jelly-like substance extracted from mouse tumour cells that allows the cells to assemble into 3D structures. Griffith wanted to put epithelial cells, which compose the uterine lining, with stromal cells that support that lining. In the body, these cells need to communicate with each other to bring about the changes that occur with the monthly cycle. But Matrigel is packed with proteins that can hamper the cell-to-cell communication. So Griffith and her colleagues developed a hydrogel that’s entirely synthetic.Griffith’s team has also been working on the next step, a model of abnormal endometrial tissue that the researchers can use to test therapies for the condition. Because blood vessels are crucial to maintaining this tissue, Griffith knew she wanted to include them. To do this, she and her colleagues placed the organoid on a microfluidic chip surrounded by cells that form blood vessels. “We put all of these cells in together at the beginning in a gel, and the blood vessels form spontaneously,” she says. “So the organoids turn into lesion-like structures,” she adds. “It’s actually kind of wild.”Griffith and her team have created these model systems from the cells of about a dozen people with endometriosis, and they’re beginning to use them to test compounds that could be promising therapies for the condition.Turco, meanwhile, has developed her endometrial organoid into a model of menstruation. Her team treated the endometrial organoids with hormones to mimic what happens when the endometrial lining is regenerating. Then the researchers stopped the hormones to mimic the start of menstruation. In the uterus, the lining breaks up naturally. In the model, however, the researchers break the organoids up mechanically. When the cells are put back into a gel, the organoids reform. “And you can keep doing this over and over again,” she says.The model allows them to study the mechanisms at work during regeneration. “That’s not possible to study in humans — like ever,” Turco says. Researchers have long thought that the stem cells that lie beneath the surface of the lining are solely responsible for regenerating it. But Turco’s research suggests that cells on the surface might have a role, too.The vagina, ovaries and moreFor Kathryn Patras, a microbiologist at Baylor College of Medicine in Houston, Texas, organoids are a way to explore the diversity of bacteria that colonize the vagina and how they influence human health. A healthy vaginal microbiome can help to prevent harmful bacteria from taking over. A disrupted microbiome, however, seems to increase a woman’s risk of catching a sexually transmitted infection and of experiencing complications during pregnancy.The vaginal microbiome is particularly tricky to study in mice. Its composition is entirely different from that of humans. And introducing a human microbiome into the mouse vagina is nearly impossible. Patras tried for years. “It just failed splendidly,” she says.So Patras and her colleagues harvest naturally existing stem cells from the human vagina and coax these cells to form organoids. These mini-vaginas are hollow balls, not tubes. And because the researchers are trying to study the vaginal lining, which isn’t spherical, they break up the organoids to make “open-faced tissue layers”, says Patras. On one side, the cells have media that nourishes them. On the other, “they’re seeing air, which is what they would see in the human tissue,” she says.One of the team’s goals is to look at whether beneficial microorganisms that are found typically in the vagina, such as Lactobacillus, can protect the vaginal tract from being colonized by harmful microbes. Although the assumption has long been that the pathogens that cause urinary tract infections come from the gut, some research suggests that the vaginal microbiome could play a part. Preventing colonization there might reduce the risk of infections in the urinary tract.Ovaries are also getting the organoid treatment, both for studying fertility and the transition to menopause, which comes with a host of aggravating symptoms and an increased risk of heart disease, stroke and osteoporosis.Francesca Duncan, a reproductive biologist at Northwestern University’s Feinberg School of Medicine in Chicago, Illinois, and her colleagues are using ovarian organoids to study reproductive ageing. Researchers in this field have focused conventionally on the ovary’s follicle. “That’s the kind of functional unit,” says Duncan. It’s the part that generates hormones and contains the developing egg. About a decade ago, however, researchers in her lab discovered that, in mice, it’s not just the egg that ages — the ovary becomes inflamed and stiffer with age. She suspects that this ovarian ageing could influence both the number and quality of the eggs and, therefore, affect fertility.Duncan wanted an in vitro model to study this ageing process and whether drugs might be able to reverse it. Plenty of labs have managed to grow follicles outside the ovary. They’ve even managed to get those follicles to give rise to eggs. But Duncan wanted to study the other cells that make up the ovary. When a graduate student suggested trying to grow an ovarian organoid, Duncan was sceptical. “It seemed like a fad,” she says. But the student was so enthusiastic that Duncan gave the project the green light. The research has already been “really, really fruitful”, she says.So far, Duncan’s team has created ovarian organoids from the ovaries of mice and rhesus macaques, finding, for example, that the stiffening of individual cells in the ovary might be responsible for how the ovary tissue stiffens as it ages.The team’s next step is to develop human ovarian organoids to screen compounds that could stave off this stiffening or even reverse it, Duncan says.Researchers are also using organoids to study ovarian cancer, the fifth-leading cause of cancer-related deaths in women. Some teams are studying how the disease emerges by examining organoids that mimic the fallopian tube. That’s because research suggests that the vast majority of the deadliest ovarian cancers actually originate there. Other groups are modelling ovarian and other cancers of the female reproductive tract by growing organoids from tumour tissue that has been taken from people with the disease.Although researchers are learning a great deal from organoids that represent a single tissue or cell type, some teams are hoping to learn even more by combining them with other organoids or incorporating them into more-complex systems. Endometrial organoids can be combined with placental organoids to study a fuller picture of invasion, for example. Or they can be mixed with lab-created embryo models to study implantation.Even these more-intricate organoids won’t capture the full complexity of human tissue. But they don’t have to. Organoids might be a reductionist model, but “still they’re revealing so much,” Turco says. “I keep getting surprised.”This article is reproduced with permission and was first published on September 23, 2025.
